An illuminated respiratory activity monitoring system identifies priming-active compounds in plant seedlings

Abstract Background Growing large crop monocultures and heavily using pesticides enhances the evolution of pesticide-insensitive pests pathogens. To reduce pesticide use in cultivation, application priming-active compounds (PrimACs) is a welcome alternative. PrimACs strengthen plant immune system could thus help to protect plants with lower amounts pesticides. can be identified, for example, by their capacity enhance respiratory activity parsley cells culture as determined oxygen transfer rate (OTR) respiration monitoring (RAMOS) or its miniaturized version, µRAMOS. The latter was designed suspensions bacteria yeast microtiter plates (MTPs). So far, RAMOS µRAMOS have not been applied adult seedlings, which would overcome limitation (µ)RAMOS suspension cell cultures. Results In this work, we introduce modified analysis seedlings. novel device allows illuminating seedlings records each well 48-well MTP. validate suitability setup identifying PrimAC Arabidopsis thaliana , were grown MTP seven days treated known salicylic acid (SA; positive control) candidate methyl 1-(3,4-dihydroxyphenyl)-2-oxocyclopentane-1-carboxylate (Tyr020). Twenty-eight h after treatment, elicited flg22, 22-amino peptide bacterial flagellin. Upon elicitation, monitored. evaluation OTR course reveals Tyr020 likely PrimAC. priming-inducing confirmed molecular biological analyses A. Conclusion We disclose difference during night period between primed unprimed distinguishable elicitation flg22. Thus, it has shown that used reliable screening